【摘要】 目的 :研究猴头菌甾醇类化合物 ,探讨醇提和水提浸膏疗效差异的药化基础。方法 :利用GC MS方法对猴头菌甾醇类物质组成进行分析 ,并采用气相色谱、反相HPLC及其他生化分析手段比较了猴头菌及其 2种方法提取物的成分。结果 :醇提浸膏较水提浸膏在粗多糖、水溶性蛋白、脂肪酸方面没有明显优势 ,但醇提与水提浸膏在甾醇类物质含量上存在显著差异。在猴头菌丝体中存在含量较高的不同双键形式的麦角甾醇类化合物、β 谷甾醇和 4个双键的C2 8甾醇化合物。结论 :猴头菌甾醇类化合物主要存在于醇提浸膏中 ,其中麦角甾醇含量最高。同时首次发现猴头菌含有麦角甾 8(14) 烯 3β 醇、β 谷甾醇和 4个双键的C2 8甾醇化合物。更多还原

【Abstract】 Objective: To study the pharmaceutic chemical basis of the different medicinal effects, and to compare and analyze the sterols in Hericium erinaceus mycelia and its ethanol extract ,water extract derived from solid fermented mycelia. Method: The components of Hericium erinaceus mycelia and two kinds of extract were compared with some biochemical methods such as GC, RP HPLC, etc. Sterol composition in Hericium erinaceus mycelia was determined by using GC mass spectrometry. Results: The content of crude polysaccharide, water soluble protein and fatty acid of ethanol extract showed no obvious advantage over its water extract. However, there was significant difference between two kinds of extract in the content of sterols. And ergostane derivatives, β sitosterol and C 28 sterol with four bonds were detected as the major sterols in Hericium erinaceus mycelia, among which ergosterol was the principal sterol. Conclusion: Sterols in Hericium erinaceus exist mainly in ethanol extract. And three sterols are found for the first time from Hericium erinaceus , which are ergostane derivatives,β sitosterol and C 28 sterol with four bonds.更多还原