【摘要】 目的 :研究黄芩苷药物代谢产物的化学结构。方法 :中药化学与现代仪器分析相结合。结果 :从口服黄芩苷的人尿液中 ,发现并鉴定了 3个主要代谢产物的化学结构。结论 :黄芩苷苷元是主要药物代谢产物的中间体 ,他们在体内共存 ,构成黄芩苷的药效。更多还原

【Abstract】 Objective:We are interested in the metabolism of baicalin in human body to offer more information to clinic.Method:Urine of ten persons was collected during 2～18 h after taking 1.0 g of baicalin orally, and the urine was concentrated to 0.5 L,then filtered, the solution being loaded on an iron exchange column eluted with 1.0 L of H 2O and 1.0 L of 50% EtOH. The EtOH fraction was concentrated and loaded on a Sephadex LH 20 column eluted with 0.5 L of H 2O to obtain 15 fractions, which were detected by silica gel TLC(CHCl 3 MeOH H 2O, 1∶1∶0.1). The fractions 8～11 showed flavonoid characteristics by spraying FeCl 3 reagent, and the four fractions were combined to obtain the sample containing metabolites of baicalin for HPLC analysis and LCMS measurement.Results and Conclusions: M 1,M 2 and M 3 was identified as baicalein 6 O β glucopyranuronoside; 6 O methyl baicalin 7 O β glucopyranuronoside and baicalin.更多还原