【摘要】 目的　观察破骨细胞抑制剂———降钙素 (密钙息 )对体外培养成骨细胞的作用。方法在新生SD大鼠头颅骨第二继代成骨细胞 (OB2 )培养液中分别加入不同浓度 (10 - 4 ～ 10 - 1 2 g ml)的密钙息 ,分别观察OB2 的增殖功能 (用波长 5 70nm处OD值表示 ) ,分化功能 [用碱性磷酸酶 (ALP)活性表示 ]和矿化功能 (用矿化结节数量 视野表示 )。结果　OD值 (均值 +标准差 )为 0 32 3± 0 10 1～0 5 2 3± 0 15 8;ALP活性 (均数±标准差 )为 (0 10 4± 0 0 12 )U mg蛋白质 ;矿化结节数量 视野 (均数±标准差 )为 5 75± 0 95 7个。结论　与对照组比较 ,适当浓度 (10 - 8～ 10 - 1 2 )的密钙息对OB2 的增殖 ,分化和矿化功能均具有促进作用 ,10 - 1 0 g ml浓度密钙息作用尤其显著 ,它们的作用显著性差异为 :促进OB2 增殖功能P <0 0 5 ;促进OB2 分化功能P <0 0 1和促进OB2 矿化功能P <0 0 0 1。更多还原

【Abstract】 Objective To observe the effects of calcitonin(Miacalcic),an inhibitor of osteoclasts,on osteoblasts in vitro. Methods The culture medium with different Miacalcic concentrations(10 -4 ～10 -12 g/ml) and the second generation osteoblasts(OB-2) from the skull of newborn SD rats were mixed for the observation respectively on the proliferation(OD value at wavelength 570nm),the differentiation(ALP activity)and the mineralization(mineralized nodes/field of vision)of the OB-2. Results The OD values(mean and standard deviation)were 0^323±0^101～0^523±0^158;the ALP activity(mean and standard deviation) was 0^104±0^012 U/mg proteins,and the mineralized nodes(mean and standard deviation)were 5^75±0^957. Conclusion In comparison with control groups,the proliferation,differentiation and mineralization of the OB-2 were promoted by calcitonin concentration(10 -8 ～10+{12}g/ml),in which the effect of 10～10 g/ml calcitonin concentration on OB2 was the most significant for promoting proliferation( P <0 05),differentiation( P <0 01) and mineralization( P <0 001).更多还原