【摘要】 目的　建立高效液相色谱法测定血浆中葫芦素B浓度的方法 ,研究葫芦素注射剂在小鼠体内的药动学。方法　采用高效液相色谱法 ,色谱柱 :HypersilODSC18,流动相 :甲醇 水 (72 :2 8) ,筛选雌酚酮作内标。样品经萃取纯化后进样 ,检测波长 2 2 8nm ,按内标法定量测定。结果　标准曲线在 0 .5～ 15 μg·mL-1内有良好线性关系 (r =0 .996 2 ) ,方法回收率在 94.11%～99 .77% ,检测限 4ng ,小鼠血浆中最低检测浓度为 5 0ng·mL-1。日内RSD小于 12 .12 % ,日间RSD小于 15 .6 3%。葫芦素水针剂小鼠静脉给药后体内的药 时过程符合二室模型。结论　首次建立的高效液相色谱法测定葫芦素B血药浓度的方法简便 ,快速准确 ,灵敏度高 ,适用于测定血浆中葫芦素B浓度及体内的药动学研究。更多还原

【Abstract】 OBJECTIVE The pharmacokinetics of cucurbitacins in mice was studied, and the HPLC method was established for the separation and quantitative determination of cucurbitacins B in plasma.METHOD Column: Hypersil ODS C 18 (150 mm×4.6 mm, 5 μm); column temperature: 30℃;mobile phase: a mixture of methanol(MeOH) and water(72∶28); flow rate: 0.5 mL·min -1 ; detection wavelength: 228 nm; aliquot injected into the HPLC system: 20 μL.RESULTS The recovery of the assay was 94.11%～99.77% with the intra-day and inter-day RSD less than 12.12% and 15.63%,respectively.The detection limit was 4ng. The lowest detection concentration was 50 ng·mL -1 in plasma.CONCLUSON This method was found to be simple, rapid ,sensitive and accurate for the determination of CuCB in plasma.更多还原