【摘要】 目的　克隆中国人谷胱甘肽过氧化物酶 (GPx1)的cDNA。方法　用逆转录 聚合酶链反应 (RT PCR) ,以中国人胎盘组织总RNA为模板 ,扩增中国人谷胱甘肽过氧化物酶 (GPx1)的cDNA ,进行序列分析。计算机分析其二级结构并比较已发现的人GPx家族的 5种GPx氨基酸序列。结果　从中国人胎盘组织中克隆的GPx1基因 ,与国外文献报道相比 ,只有 1个氨基酸残基发生变异 ,即leu2 2 变为Val2 2 ,该变化不影响GPx1活性中心的结构 ;人GPx的硒结合区及活性中心区域的氨基酸序列是高度保守的。结论　首次克隆了中国人体特异的胞质内GPx1基因 ,为其生物学活性及结构与功能的关系的研究创造了条件。更多还原

【Abstract】 OBJECTIVE To clone the cDNA of human glutathione peroxidase 1(GPx1).METHODS The total RNA was isolated from normal Chinese human placenta and was amplified as the template by RT PCR.It was inserted into pSK plasmid to analyze its sequence;the sequences of 5 known GPx gene were compared.RESULTS The GPx1 cDNA from placenta had 1 base pare and 1 amino acid residue (Leu 22 mutated to Val 22 ),different from that of reported.But it didnt alter the secondly structure of GPx selenocysteint residue and the active sites were highly conserved in 5 GPxs.CONCLUSION The human GPx1 gene of Chinese was cloned for the first time.更多还原