【摘要】 目的　建立高效液相色谱法测定丹参酮ⅡA 含量的方法。方法　C18柱以甲醇 水 (70∶30 )作为流动相 ,检测波长 2 6 9nm。结果　丹参酮ⅡA 浓度在 0～ 33ng·mL-1范围内 ,峰面积与进样量线形关系良好 ,回归方程Y =972 5X- 2 5 84,r =0 .9997。平均回收率为 98.2 9% ,RSD =1.86 %。结论　本法重现性好 ,准确快速 ,可作为质量控制指标更多还原

【Abstract】 OBJECTIVE To develope an HPLC assay for the determination of tanshinoneⅡ A.METHOD C 18 ODS column was used.The mobile phase was consisted of MeOH water(70∶30).The detection wavelength was at 269 nm.RESULT The linear regression equation was Y=9725X-2584,r=0.9997.The average recovery was 98.29% with RSD =1.86%.( n =5).CONCLUSION The method may be used for quality control.更多还原