【摘要】 目的 :探讨碱性成纤维细胞生长因子对体外培养的中脑多巴胺能神经元的作用。方法 :在培养孕 14～16d大鼠胚胎黑质神经元中加入碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF) ,不同时间对培养细胞进行观察并计数存活的黑质细胞总数及长神经突起细胞总数。结果 :培养 1周后bFGF可促进多巴胺能神经元的存活和生长。结论 :bFGF对体外培养的多巴胺能神经元有营养作用更多还原

【Abstract】 Objective: Our aim was to study the nutrient effect of basic fibroblast growth factor(bFGF)on dopaminergic (DA) neurons of the mensencephalic subtantia nigra in vitro. Methods:Subtantia nigra neurons from rats E14 to 16 were cultured with bFGF and observed by using light microscopy. Results:The bFGF can promote the development of mesencephalic DA neurons after one week. Conclusion:The bFGF has neutrient effect on DA neurons of subtantia nigra in vitro.更多还原