【摘要】 目的 :检测SLE患儿谷胱甘肽转移酶 μ(GSTμ)基因缺失程度及血中一氧化氮 (NO)、脂质过氧化物(LPO)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH px)和谷胱甘肽 (GSH)含量 ,分析其与SLE发病之间的关系。方法 :用PCR法检测 2 2例SLE患儿和 2 3例健康对照儿童的GSTμ基因 ,用化学分析法测上述 5项指标。结果 :SLE患儿GSTμ基因缺失率达 90 .91% ,明显高于对照组的 5 6.5 2 %。SLE活动期NO( 77.4 3± 15 .19)μmol/L、LPO( 9.95± 1.84 ) μmol/L ,明显高于稳定期和对照组的水平。SLE活动期SOD( 12 2 0 .76± 3 0 2 .81) μU/L、GSH -px( 80 .0 4± 2 4 .4 5 )U/mg、GSH( 0 .4 1± 0 .0 5 )mg/g ,明显低于稳定期及对照组水平。在SLE稳定期GSH( 0 .95± 0 .2 0 )mg/g ,仍明显低于对照组水平。血清NO水平与LPO呈直线正相关 ,与SOD、GSH Px、GSH呈直线负相关。抗ds DNA与NO、LPO呈直线正相关。结论 :GSTμ基因缺失可能是SLE发病的遗传因素之一 ,SLE活动期患者存在氧化损伤及抗氧化能力下降 ,且NO、LPO、SOD、GSH px可作为判断SLE病情变化的一组重要指标 ,提示对SLE患儿应给予抗氧化治疗。目的 :检测SLE患儿谷胱甘肽转移酶 μ(GSTμ)基因缺失程度及血中一氧化氮 (NO)、脂质过氧化物(LPO)、超氧化物歧化酶更多还原

【Abstract】 Objective: Our aims were to determine the glutathione S transferase class μ(GSTμ) gene and the levels of nitric oxide (NO),lipid peroxide(LPO),superoxide dismutase(SOD),glutathione peroxidase(GSH px), and glutathione(GSH) of children with systemic lupus erythematosus(SLE) and to analyze the relationship between these parameters and SLE. Methods: The GSTμ gene of 23 SLE patients and 23 healthy children were determined by PCR method. The NO,LPO,SOD,GSH px, and GSH were determined by chemical analysis methods. Results: The rate of GSTμ gene deletion in the cases of SLE was 90.91%,which was significantly higher than that of the controls(56.52%).The levels of NO(77.43±15.19μmol/L) and LPO(9.95±1.84μmol/L) of active SLE group were higher than those of stable SLE group (NO 56.01±12.53μmol/L,LPO 6.39±1.70μmol/L) and healthy group (NO 51.06±7.99μmol/L,LPO 5.87 ±1.40μmol/L).The levels of SOD(1220.76±302.81μU/L),GSH px(80.04±24.45 U/mg), and GSH(0.41±0.05mg/g) of active SLE group were lower than those of stable SLE group(SOD 1985.30±391.22μU/L,GSH-Px 136.55 ±18.79 U/mg,GSH 0.95±0.20mg/g) and healthy group(SOD 2110.12±384.15μU/L,GSH-Px141.37± 28.43 U/mg,GSH 1.26±0.21mg/g).The level of GSH(0.95±0.20)mg/g of stable SLE group was lower than that of healthy group(1.26±0.21mg/g).An obvious positive correlation existed between the serum NO and LPO(r=0.720; P<0.01).Obvious negative correlations existed between NO and SOD,GSH px,GSH(r=-0.757,-0.774,-0.691; P<0.01).The obvious positive correlations were observed between anti ds DNA and NO,LPO(r=0.773,0.695; P< 0.01 ).The obvious negative correlations were observed between anti ds DNA and SOD,GSH px,GSH(r=-0.614, -0.766 ,-0.701; P<0.01). Conclusion: The GSTμ gene deletion may play an important role in etiopathology of SLE.There are oxidative injury and lower anti oxidation function in active SLE. The NO,LPO,SOD,and GSH-px may be important indicators for determining the conditions of the disease.They also provide theoretical and experimental basis for the application of anti oxidation therapy in SLE.更多还原