【摘要】 目的 :研究干扰素 αⅡb(IFN αⅡb)单独以及与卡铂联合应用时 ,在体外对卵巢上皮癌细胞株OVCAR 3和HO 8910增殖的抑制作用。方法 :应用四唑蓝显色法 (MTT法 ) ,检测IFN αⅡb以及联合应用卡铂作用后OV CAR 3和HO 8910细胞的增殖变化。结果 :(1)当IFN αⅡb的浓度≥ 10 3 IU/ml时 ,对OVCAR 3和HO 8910细胞的增殖均具有一定的抑制作用 ,而且抑制作用与浓度呈正相关 (P <0 0 1)。(2 )各浓度的IFN αⅡb和卡铂联合应用时 ,未见协同作用。结论 :干扰素具有抗卵巢上皮癌增殖作用更多还原

【Abstract】 Objective: Our aim was to evaluate the inhibitory effects of interferon (IFN-αⅡb) alone or in combination with carboplatin on the proliferation of two cell lines of human epithelial ovarian carcinoma, OVCAR-3 and HO-8910. Methods: After exposure to various concentration of IFN-αⅡb and carboplatin, the viability of the cell lines of human ovarian epithelial carcinoma was measured by using a tetrazolium salt, MTT assay which was converted to blue formazan crystals by living cell. The amount of formazan was quantitated by using a microtitre plate spectrophotometer. Results: The OVCAR-3 and HO-8910 were inhibited to different degrees when the concentration of IFN-αⅡb alone was 105U/ml, 104U/ml, and 103U/ml, and IFN-αⅡb inhibited the proliferation of these cells in a dose-dependent manner (P<0.01). Carboplatin did not show any synergistic effect on HO-8910 and OVCAR-3 cell lines when combined with IFN-αⅡb (P>0.05).Conclusion: Interferon α-Ⅱb have the potential effect of inhibiting the proliferation of human ovarian carcinoma.更多还原