【摘要】 目的 :在大肠杆菌中克隆肺炎支原体P1蛋白结构基因 ,为实现肺炎支原体P1蛋白结构基因的大量扩增及表达奠定基础。方法 :PCR方法获取肺炎支原体P1蛋白结构基因 ,并与 pUC19载体DNA连接 ,转入大肠杆菌JM10 9菌株。X -Gal平板筛选转化子 ,用PCR方法和限制性核酸内切酶酶切图谱分析方法鉴定重组克隆。结果 :PCR和限制性核酸内切酶图谱分析均证实所获重组质粒中含有P1蛋白基因。结论 :获得了含有肺炎支原体P1蛋白结构基因的克隆菌株。更多还原

【Abstract】 Objective: Our aim was to clone P1 protein structure gene of mycoplasma pneumoniae in escherichia coli. That will be a foundation for amplification and expression of P1 protein gene.Methods:The P1 protein gene was acquired by PCR method, and we ligated it together with pUC19 vector DNA. Then it was transformed into E.coli JM109. The recombinants were screened by X-Gal plate, and identified by PCR method and endonuclease map analysis method.Results:Recombinant plasmid was proved to carry P1 protein gene by using PCR and endonuclease map analysis method.Conclusion:We obtained the recombinant clone strain that contain P1 protein structure gene of mycoplasma pneumoniae .更多还原