【摘要】 目的 探讨埃尔托型霍乱弧菌(EVc)在流行间歇期的保菌越冬及流行期的复苏,为霍乱的防治提供依据。方法 运用电镜、荧光显微镜、普通显微镜等检测方法,对埃尔托霍乱弧菌在模拟越冬中的超微结构进行了观察研究。结果 研究发现,进入低温低营养条件后,EVc在模拟水体中以纤细状体和正常弧菌的交替生长形式存在,在此过程中,大部分细菌自溶,向环境提供营养成分,少部分通过潜-生序的变化得以存活,细菌群体在两种形式的演变中显示一定的有序性。纤细状体与细菌体的交替变化通过一系列的变异形式,包括圆球状体、巨形体、原生小体和革兰阳性小细胞等。结论 本实验不仅对霍乱弧菌的越冬机理作出解释,而且观察到革兰阴性菌在不利条件下的一种细胞多分裂增殖方式,这种方式在自然界中可能普遍存在。更多还原

【Abstract】 Objective To explore the overwintering mechanism of Evc. Methods We observed EVc’ s survival process during simulated ovenvintering in the acmatic environment by microscope, fluorescope and electron microscope.Results The results was con-firmed in water environment, the EVc altemated between tlireadsliaped form and bacterial form .Some celis died and other survived through latent- growth sequence.The sequence variant included threadshaped form, sphericol bodies, large bodies, sniall dense parti-cles, small grani positive celi. Condusions We observed a new multiplicative division pattem of bacterial crvptic growth celis, it may be widespread existence of gram negative cell in nature.更多还原