【摘要】 目的 :建立一种简便、快速测定血浆中甲醇、乙醇浓度的顶空气相色谱法 ,并与直接进样法测定结果进行比较。方法 :外标法定量 ,分析柱为GDX -102玻璃填充柱 ,载气为N2,检测器为FID ;0.5ml血浆样品以小瓶密封 ,25℃下平衡20min ,用气密性注射器取顶空进样1.0ml。结果 :本法甲醇和乙醇的线性范围为0.2～7.5g/L ;日内RSD<4 % ,日间RSD<5.5 % ;方法回收率甲醇为(100.9±3.8) % ,乙醇为 (101.5±3.4) % ;最低检测限<0.01g/L。结论 :本法操作简便、快速 ,灵敏度高 ,结果准确可靠更多还原

【Abstract】 OBJECTIVE:To establish a HS-GC method for determining methanol and ethanol in human plasma METHOD_S:An external standard method was used The stationary phase was a glass column packed with GDX-102 The carrier gas was nitrogen The column temperature was 140℃ and both the injector temperature and the detector temperature were 200℃ RE_SULTS:A good linearity was obtained in the range of 0 2～7 5g/L of methanol and ethanol The within-day RSD was less than 4% and the between-day RSD less than 5 5% The recoveries were (100 9±3 8)% for methanol,(101 5±3 4)% for et_hanol,respectively The limits of detection for both methanol and ethanol were less than 0 01g/L CONCLUSION:This method is simple,rapid and precise更多还原