【摘要】 目的 :应用反相高效液相色谱法同时测定哌拉西林和他唑巴坦的含量。方法 :以咖啡因为内标 ,μBondapakC18 为固定相 ,甲醇 -水 (34 5∶65 5)为流动相 ,检测波长为220nm ,流速为1 0ml/min。结果 :哌拉西林的线性范围为40～200μg/ml(r=0.9996,n=6) ,他唑巴坦的线性范围为5 0～25 0μg/ml(r=0.9991,n=6) ,平均回收率及RSD (n=5)为哌拉西林 (100 2±0 61) % ,他唑巴坦 (99 03±0 90) %。结论 :本法简便、快速、准确更多还原

【Abstract】 OBJECTIVE:To establish a RP-HPLC method to simultaneously determine the contents of piperacillin and taz_obactam METHODS:Using μ Bondapak C18 column as fixed phase,a mixture of methanol and purified water(34 5∶65 5)as mobile phase,and caffeine as internal standard,detecting wavelength:220nm,flow rate:1 0ml/min RESULTS:The linear rangs of piperacillin and tazobactam were 40～200μg/ml and 5 0～25 0μg/ml respectively The average recovery and RSD (n=5) were (100 2±0 61)% for piperacillin and (99 03±0 90)% for tazobactam CONCLUSION:This method is rapid,simple and accurate更多还原