【摘要】 EIAV在繁殖过程中涉及到多种转录因子的调节 ,其中Rev蛋白是病毒编码的转录后调节因子 ,决定了病毒复制由早期到晚期的过渡。Rev是非结构蛋白 ,由病毒基因组全长转录产物经多次拼接而成。本研究使用EIAV疫苗毒感染驴巨噬细胞培养物 ,在病毒增殖早期提取总RNA ,反转录后使用EIAV特异引物扩增病毒基因组拼接产物。将扩增产物克隆后经过核苷酸序列分析 ,通过与基因组全序列的比较 ,确定了编码Rev蛋白的病毒基因组转录产物的编码序列和拼接位点更多还原

【Abstract】 Replication of EIAV is regulated by several transcription factors.Rev is encoded by multispliced genomic mRNA,which switches the virus gene expression from early stage to late stage.In this study,donkey macrophage culture was infected with donkey leukocyte attenuated EIAV;total cellular RNA was extracted from cells at the early stage of the infection.After reverse transcription directed by EIAV specific primer,polymerase chain reaction was performed.Then the amplified segment was cloned and sequenced.By comparison with the viral genome,ORF of rev and splicing sites were determined.更多还原