【摘要】 应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。更多还原

【Abstract】 In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG（rBCG）expressing MalE protein of Escherichia coli （rBCG·MalE）,the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL₂ responses to MalE protein or its peptides in enzyme linked immunospot （ELISPOT）assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE were CD4 + T cell_dependent.The results of analyzing T cell responses to MalE protein or PPD in different time points ofter rBCG·MalE immunization showed that initial Thl responses to rBCG or BCG were modulated during the course of infection to become a mixed Th1/Th2 responses.These results revealed new mechanisms in T cell immune responese against mycobacteria,and further showed that BCG is an effective live vector to express and deliver foreign antigens to host immune system.更多还原