【摘要】 研究不同浓度的金属离子及有机物对蜡样芽胞杆菌蛋白酶活力的影响 ,结果表明 :1五种无机离子中 Mg2 +、Na+、K+对酶有激活作用 ,其中 Mg2 +在 2× 10 - 2 mol/ L 浓度激活作用最大。随浓度降低 ,Mg2 +和 K+ 对酶的激活作用均减弱。Ca2 + 和 Zn2 + 对酶活力有抑制作用。2在七种重金属离子中 ,除 Mn2 + 对蛋白酶有激活作用外 ,Cu2 + 、Pb2 + 、Cd2 + 、Co2 + 、Cr3+ 和 Cr6 + 均为抑制作用 ,而且随浓度降低 ,抑制作用均减弱。3在有机物中 ,半胱氨酸和 EDTA对酶均表现激活作用 ,且在 2× 10 - 2 m ol/ L 最为强烈。 4Na+ - K+ 、Ca2 + -Mg2 + 和 Cu2 + - EDTA共同作用时对蛋白酶活力均有不同程度的抑制作用更多还原

【Abstract】 The effects of some ions and organic compounds on protease activities of Bacillus cereus were tested. The results were as follows:① Mg 2+ , Na + and K + were showed to activate the protease activities among five inorganic ions and Mg 2+ had the most intensive effect on protease at 2×10 2 mol/L. The impacts of Mg 2+ and K + declined with their concentrations respectively. Zn 2+ and Ca 2+ inhibited the protease activities.② All seven heavy metal ions were shown to have inhibition except Mn 2+ which activated the protease at three concentrations and the suppressed effects were decreased with concentrations.③Both cysteine and EDTA had the activated effects on protease activities and the effects were most remarkable at 2×10 2 mol/L.④The combinations of Na + and K + ,Ca 2+ and Mg 2+ , EDTA and Cu 2+ all inhibited the protease activities to some extent.更多还原