【摘要】 为优化从酸奶中分离保加利亚乳杆菌的培养条件 ,初步建立一种较简单有效的分离方法。以成都市市售的主要酸奶——华西牌瓶装酸牛奶为来源 ,采用 MRS、SL、L S、番茄酵母 4种经典的乳酸菌选择性培养基 ,并分别进行有氧及烛缸厌氧分离培养测定其得率。采用 X2 检验对不同组的分离得率进行统计学分析。结果表明 MRS培养基为酸奶中保加利亚乳杆菌分离首选培养基 ,分离得率达 6 6 .6 7% ;微氧环境优于有氧环境 (P<0 .0 5 )。更多还原

【Abstract】 To optimize the condition of separating method,this study used MRS,SL,LS and tomato-yeast these 4 Lactobacillus selectivity culture mediums to separate the Lactobacillus bulgaricus with or without aeration respectively from Chengdulocal yogurt-Hua Xi brand yogurt,and determine the separating positive rate respectively.Using X2 test to analyze these rates,the results showed that:MRS culture medium was the opimum culture medium toseparate the Lactobacillus bulgaricus from yogurt,its separating positive rate was 66.67%;separating with aeration is better than without aeration(P<0.05).更多还原