【摘要】 以伪狂犬病病毒 (PRV)HB 93 0 4株的基因组为模板 ,利用聚合酶链反应 (PCR)对其gG gD基因片段进行扩增 ,获得了预定大小的片段 ,将这一片段克隆到质粒载体 pPK中。对重组质粒pPKGD进行限制性内切酶分析、PCR鉴定和克隆片段的序列测定 ,证实了克隆片段的可靠性。更多还原

【Abstract】 Using the genome DNA of the strain HB 9304 of Pseudorabies virus (PRV) as template, the fragments flanking gG and gD were amplified by polymerase chain reaction(PCR) .The expected size of fragments were obtained ,and then cloned into the plasmid pPK. The recombinant plasimd of pPKGD was identified by restriction enzyme analysis,PCR and sequencing, which proved completely its validity.更多还原