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兰州地区实验动物肺支原体感染调查和分离鉴定

Surveys on Mycoplasma pulmonis Infection of Laboratory Animals in Lanzhou and Isolation and Identification of the Positive Strains

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【作者】 王保宁宁磊

【Author】 WANG Baoning,NING Lei (Lanzhou Institute of Biological Products, Lanzhou 730046, China)

【机构】 卫生部兰州生物制品研究所卫生部兰州生物制品研究所 兰州730046兰州730046

【摘要】 目的 了解兰州地区啮齿类实验动物的肺支原体感染情况和感染菌株。方法 用分离培养法对兰州地区 6 40只啮齿类实验动物肺支原体的感染情况分春夏秋冬进行调查 ,并对分离株进行克隆纯化 ,从形态学 ,生化特性和血清学方面进行鉴定。结果 肺支原体在普通级小鼠中的感染率为 2 3% ,普通级豚鼠、地鼠、大鼠和清洁级小鼠未发现有感染 ,且感染率与季节无明显相关性。分离株经鉴定均为支原体科支原体属肺支原体

【Abstract】 Objective To survey the infectious rate and strain of M.pulmonis in the rodent laboratory animal in Lanzhou districton. Methods The M.pulmonis infection of 640 rodents labovatory animals in Lanzhou were surveyed by culture method. The isolates were cloned and identified by morphology, biochemical features and serology. Resultsd and conclusion The infectious rate of M.pulmonis was 23 % in the conventional mice.No infection were fond in rat, guinea pig and hamster. There was no seasonal correlation to M.pulmonis infection in the mice. According to theose experiments, the isolates were Mycoplarma pulmonis, Mycoplasmataceac, Mycoplasmatales.

【关键词】 肺支原体感染率分离鉴定
【Key words】 M.pulmonisInfectious rateIsolation and identifiation
  • 【文献出处】 中国实验动物学报 ,Acta Laboratorium Animalis Scientia Sinica , 编辑部邮箱 ,2001年04期
  • 【分类号】S852.62
  • 【被引频次】5
  • 【下载频次】65
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