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丹参注射液对β2糖蛋白Ⅰ诱导产生抗心磷脂抗体的影响(英文)

Effect of Salvia miltiorrhiza Bunge injectio on anticardiolipin antibody production induced by β2 glycoprotein I

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【作者】 陈刚刘润侠张王刚王彬付杰冯义国张小斌吴峰马西

【Author】 CHEN Gang, LIU Run-Xia, ZHANG Wang-Gang, WANG Bin, FU Jie, FENG Yi-Guo, ZHANG Xiao-Bin (Department of Hematology, Second affiliated Hospital, Xi-an Jiaotong University, Xi-an 710004, China ) WU Feng, MA Xi (Institute of Experimental Hematology, Xi-an Jiaotong University, Xi-an 710061, China)

【机构】 西安交通大学第二医院血液内科西安交通大学实验血液研究所西安交通大学实验血液研究所 西安中国 710004西安中国 710061中国 710061

【摘要】 目的:观察丹参注射液对β2糖蛋白Ⅰ(β2-GPⅠ)诱导的抗心磷脂抗体(anticardiolipin antibody,aCL)产生的影响,以探讨其对抗磷脂综合征的治疗作用及可能的作用机制。方法:60只雌性昆明种小鼠被随机分为六组,A、B、C、D四组经腹腔注射不同剂量的丹参注射液;14天后,A、B、C、E四组多部 位皮下注射β2-GPⅠ。E组为造模组;F组为空白对照组。用酶联免疫吸附实验法测定血清中的aCL;以链霉亲和素-生物素复合物法检测外周血T细胞亚群;以四甲基偶氮唑盐(MTT)比色法检测外周血白细胞介素-2(IL-2)生物活性。结果:(1)用药组血清aCL吸光度值均较E组有不同程度降低(P<0.05或P<0.01);药物剂量与aCL吸光度值的变化成负相关(P<0.01)。(2)与E组相比,A、B、C组均可降低TH/Ts比值(P<0.05或P<0.01);D组与F组相比无统计学意义(P>0.05);药物剂量与TH/Ts比值存在负相关(P<0.01)。(3)与E组相比,A组对IL-2活性无明显影响(P>0.05),而B、C组则使之明显降低(P<0.01);D组与F组无明显差异(P>0.05);药物剂量与IL-2活性存在负相关(P<0.01)。(4)经双变量直线相关分析,TH/Ts比值和IL-2生物活性呈正相关(P<0.01)。结论:丹参注射液可抑制β2-GPⅠ诱导的aCL的产生,其作用机理可能是抑制TH/Ts比值和IL-2生物活性的升高;丹参注射液对?

【Abstract】 AIM: To explore the therapeutic effect and the mechanism of Chinese herbs on antiphospholipid syndrome (APS) by observing the effect of Salvia miltiorrhiza Bunge injectio ( SmBI) on anticardiolipin antibody (aCL) induced by β2 glycoprotein I (β2-GP I ). METHODS: Sixty female mice randomly fell into 6 groups; group A, B, C, D was injected through abdominal cavity with different dosage of SmBl daily; after 14 d, group A, B, C, E was immunized with 150 μg of purified human β2-GP I in complete Freund’s adjuvant subcutaneously; group F as control. The litre of aCL were detected by enzyme linked immunosorbent assay; subsets of T cell were grouped by streptavidin-biotin complex technique; and the activity of IL-2 was measured by MTT chromatometry. RESULTS: (1) Compared with group E, the absorbance (A) of aCL in group A, B, and C was decreased (P < 0.05 or P < 0.01). By linear correlation, the dosage is negatively correlated with the A values of aCL in 1, 2, and 3 weeks (P < 0.01). (2) Compared with group E, TH/TS ratio was reduced in group A, B, and C (P < 0.05 or P < 0.01); there is no significant differences between group D and F (P > 0.05). By linear correlation, the dosage is negatively correlated with TH/TS ratio (P < 0.01). (3) Compared with E, the activity of IL-2 in group B and C decreased significantly ( P < 0.01). By linear correlation, there is negative correlation between dosage and IL-2 activity ( P < 0.01). There is no significant difference between D and F (P > 0.05) . (4) There is positive correlation between TH/TS ratio and LL-2 activityin different dilutions (P < 0.01). CONCLUSION: The mechanism of suppressive effect of Sm BI on aCL induced by β2-GP I may be realized by resuming the elevated TH/TS ratio and IL-2 activity. The state that Sm BI have no effect on normal mice indicates that SmBI has selective immunoregulative functive.

  • 【文献出处】 Acta Pharmacologica Sinica ,中国药理学报(英文版) , 编辑部邮箱 ,2001年12期
  • 【分类号】R285
  • 【被引频次】2
  • 【下载频次】67
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