【摘要】 目的:观察丹参注射液对β₂糖蛋白Ⅰ（β₂-GPⅠ）诱导的抗心磷脂抗体（anticardiolipin antibody,aCL）产生的影响,以探讨其对抗磷脂综合征的治疗作用及可能的作用机制。方法:60只雌性昆明种小鼠被随机分为六组,A、B、C、D四组经腹腔注射不同剂量的丹参注射液;14天后,A、B、C、E四组多部 位皮下注射β₂-GPⅠ。E组为造模组;F组为空白对照组。用酶联免疫吸附实验法测定血清中的aCL;以链霉亲和素-生物素复合物法检测外周血T细胞亚群;以四甲基偶氮唑盐（MTT）比色法检测外周血白细胞介素-2（IL-2）生物活性。结果:（1）用药组血清aCL吸光度值均较E组有不同程度降低（P<0.05或P<0.01）;药物剂量与aCL吸光度值的变化成负相关（P<0.01）。（2）与E组相比,A、B、C组均可降低T_H/T_s比值（P<0.05或P<0.01）;D组与F组相比无统计学意义（P>0.05）;药物剂量与T_H/T_s比值存在负相关（P<0.01）。（3）与E组相比,A组对IL-2活性无明显影响（P>0.05）,而B、C组则使之明显降低（P<0.01）;D组与F组无明显差异（P>0.05）;药物剂量与IL-2活性存在负相关（P<0.01）。（4）经双变量直线相关分析,T_H/T_s比值和IL-2生物活性呈正相关（P<0.01）。结论:丹参注射液可抑制β₂-GPⅠ诱导的aCL的产生,其作用机理可能是抑制T_H/T_s比值和IL-2生物活性的升高;丹参注射液对?更多还原

【Abstract】 AIM: To explore the therapeutic effect and the mechanism of Chinese herbs on antiphospholipid syndrome (APS) by observing the effect of Salvia miltiorrhiza Bunge injectio ( SmBI) on anticardiolipin antibody (aCL) induced by β2 glycoprotein I (β2-GP I ). METHODS: Sixty female mice randomly fell into 6 groups; group A, B, C, D was injected through abdominal cavity with different dosage of SmBl daily; after 14 d, group A, B, C, E was immunized with 150 μg of purified human β2-GP I in complete Freund’s adjuvant subcutaneously; group F as control. The litre of aCL were detected by enzyme linked immunosorbent assay; subsets of T cell were grouped by streptavidin-biotin complex technique; and the activity of IL-2 was measured by MTT chromatometry. RESULTS: (1) Compared with group E, the absorbance (A) of aCL in group A, B, and C was decreased (P < 0.05 or P < 0.01). By linear correlation, the dosage is negatively correlated with the A values of aCL in 1, 2, and 3 weeks (P < 0.01). (2) Compared with group E, TH/TS ratio was reduced in group A, B, and C (P < 0.05 or P < 0.01); there is no significant differences between group D and F (P > 0.05). By linear correlation, the dosage is negatively correlated with TH/TS ratio (P < 0.01). (3) Compared with E, the activity of IL-2 in group B and C decreased significantly ( P < 0.01). By linear correlation, there is negative correlation between dosage and IL-2 activity ( P < 0.01). There is no significant difference between D and F (P > 0.05) . (4) There is positive correlation between TH/TS ratio and LL-2 activityin different dilutions (P < 0.01). CONCLUSION: The mechanism of suppressive effect of Sm BI on aCL induced by β2-GP I may be realized by resuming the elevated TH/TS ratio and IL-2 activity. The state that Sm BI have no effect on normal mice indicates that SmBI has selective immunoregulative functive.更多还原