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葡萄籽中原花青素的抗炎作用和机制(英文)

Anti-inflammatory effect and mechanism of proanthocyanidins from grape seeds

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【作者】 李文广张小郁吴勇杰田暄

【Author】 LI Wen-Guang, ZHANG Xiao-Yu, WU Yong-Jie, TIAN Xuan(Department of Pharmacology, Department of Physiology, Lanzhou Medical College; National Laboratory ofOrganic Chemistry, Lanzhou University, Lanzhou 730000, China)

【机构】 兰州医学院药理学教研室兰州医学院生理学教研室兰州大学应用有机化学国家重点实验室 兰州中国 730000兰州中国 730000

【摘要】 目的:研究葡萄籽中原花青素(PA)的抗炎作用及机制。方法:用巴豆油诱导的小鼠耳肿和角叉菜胶诱导的大鼠足肿模型,研究PA对炎症的影响。NOS活性用NADPH黄递酶染色法,NO含量用Griess重氮化反应法,β-NAG用对硝基酚比色法,MDA用TBA荧光法测定,IL-1β、TNFα和PGE2含量用放免法测定。结果:PA 10-40mg/kg ip剂量依赖性地抑制角叉菜胶诱导的大鼠足肿和巴豆油诱导的小鼠耳肿。PA 10 mg/kg减少大鼠致炎足MDA生成,抑制炎症渗出液中NOS和β-NAG活性,降低IL-1β、TNF和PGE2含量。其作用强于地塞米松2mg/kg。结论:PA对大鼠和小鼠实验性炎症有明显的抗炎作用。其抗炎机理和清除氧自由基、抗脂质过氧化和减少细胞因子的生成有关。

【Abstract】 AIM: To investigate the anti-inflammatory effect and mechanism of proanthocyanidins (PA) from grape seeds. METHODS: Croton oil-induced ear swelling in mice and carrageenan-induced hind paw edema in rats were prepared. The nitric oxide synthase (NOS) activity was measured by NADPH-diaphoras stain assay, nitric oxide (NO) content by Griess diazotization assay, N-acetyl-β-D-glucosaminidase (β-NAG ) activity by spectro-photography, malondialdehyde ( MDA) content by thiobarbituric acid (TEA) fluorescence technique, and IL-1β, TNFa, and PGE2 content by radioimmunoassay (RIA). RESULTS: PA 10 - 40 mg/kg ip inhibited carrageenan-induced paw edema in rats and croton oil-induced ear swelling in mice in a dose-dependent manner. PA 10 mg/kg reduced MDA content in inflamed paws, inhibited β-NAG and NOS activity, and lowered the content of NO, IL-1β, TNFa, and PGE2 in exudate from edema paws of rats induced by carrageenan. The inhibitory effect of PA on all above indices was more evident than that of dexamethasone 2 mg/kg. CONCLUSION: PA has anti-inflammatory effect on experimental inflammation in rats and mice. Its mechanisms of anti-inflammatory action are relevant to oxygen free radical scavenging, anti-lipid peroxidation, and inhibition of the formation of inflammatory cytokines.

【基金】 Natural Science Foundation of Gansu Provice, No ZS 001-A23-059Y
  • 【文献出处】 Acta Pharmacologica Sinica ,中国药理学报(英文版) , 编辑部邮箱 ,2001年12期
  • 【分类号】R285
  • 【被引频次】97
  • 【下载频次】472
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