节点文献
桂竹糖芥苷G对肾小管功能及髓袢升支粗段70-pS钾通道活性的影响(英文)
Effects of erysimin G on renal tubular function and 70-pS K ~+ channel activity of thick ascending limb
【摘要】 目的:研究桂竹糖芥苷G(erysimine G)的利尿作用及其作用机制。方法:应用尿道插管观察给药前后尿量变化;并应用膜片箝技术记录大鼠肾脏髓袢升支粗段70-pS钾通道的活性改变。结果:给予桂竹糖芥苷G后,实验动物尿量显著增加。单通道实验记录表明:应用桂竹糖芥苷G1μmol·L-1抑制TAL70-pS钾通道的活性,NPo由正常对照的1.67±0.10降低至0.05±0.02(P<0.01,n=4)。结论:桂竹糖芥苷G有明显的利尿作用,其利尿机制可能与抑制TAL单细胞70-pS钾通道的活性有关。
【Abstract】 AIM: To study the effect of erysimin G (CH35H52O13 on the thick ascending limb (TAL) 70-pS K+ channel of rat kidney and its effect on diuresis. METHODS: The patch-clamp cell-attached recording technique was used to record the single potassium channel current, and the urine volume (UV) was measured by urethal intubation to determine the diuretic effect. RESULTS: Erysimin G can increase the urine volume and decrease the 70-pS potassium channel activity of TAL. CONCLUSION: Erysimin G has a diuretic effect and its inhibition on the activity of apical 70-pS potassium channel may be the mechanism of its diuretic effect.
【Key words】 Erysimum cheirarithoides L; kidney; patch-clamp techniques; potassium channels;
- 【文献出处】 Acta Pharmacologica Sinica ,中国药理学报(英文版) , 编辑部邮箱 ,2001年05期
- 【分类号】R285
- 【下载频次】78