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蜂毒肽增强豚鼠心室肌细胞Na⁺-Ca²⁺交换电流（英文）

Stimulation by melittin of Na~+-Ca~2+ exchange current in ventricular myocytes of guinea pigs

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【Author】 ZHANG Xue-Mei, YANG Shen, ZHANG Yi, ZHENG Ping, JIANG Ming-Hua, ZHOU Zhao-Nian (Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031, China; State Key Laboratory of Medical Neurobiology, Medical Center of Fudan University, Shanghai 200032, China )

【机构】复旦大学药学院药理教研室；中国科学院生理研究所；复旦大学医学院神经生物学国家重点实验室；中国科学院生理研究所上海中国 200031；上海中国 200032；上海中国 200031；

【摘要】目的:研究蜂毒肽使心肌细胞钙超负荷的机制。方法:全细胞膜片箝记录。结果:蜂毒肽0.05,0.1μmol/L促进I_Na峰值（nA）从-2.1±0.8分别增加到-3.2±1.0（n=7,P<0.05）和-3.7±1.5（n=7,P<0.05）。蜂毒肽0.05,0.1,0.2μmol/L对I_Ca无显著性作用,但在箝制电位为+50mV时,促进I_Na-Ca（pA）从53±21分别增加到427±256（n=5,P<0.05）,349±147（n=5,P<0.01）和320±97（n=5,P<0.05）。结论:蜂毒肽使心肌细胞内钙增加主要是由于促进Na⁺-Ca²⁺交换电流而不是L-型钙电流。更多还原

【Abstract】 AIM:To study the mechanism of calcium overload induced by melittin in myocytes. METHODS: Whole-cell patch-clamp technique was applied for recording the currents. RESULTS: Mel 0.05, 0.1μmol/L increased the peak amplitude of INa(nA) from -2.1 ±0.8 to -3.2 ± 1.0 (n = 7, P<0.05) and -3.7±1.5 (n = 7, P < 0.05) respectively at testing potential of - 40 mV. Mel 0.05, 0.1, 0.2 μmol/L had no significant effect on ICa, but enhanced INa-ca(pA) from 53 ± 21 to 427±256 (n=5, P<0.05), 349±147 (n=5, P< 0.01) and 320±97 (n = 5, P<0.05) respectively at a testing potential of ± 50 mV. CONCLUSION: The stimulating effect of Mel on INa-Ca rather than the effect on ICa contributes to the calcium overload of myocytes.更多还原