【摘要】 :山西省卫生防疫站脊髓灰质炎 (脊灰 )实验室从 1998年 7月正式使用L2 0B细胞 ,并同时使用RD和Hep - 2细胞进行急性弛缓性麻痹 (AFP)病例病原学监测 ,为了解同时使用 3种细胞对AFP病例病原学监测结果的影响 ,将 3 0 8例AFP病例粪便标本在 3种细胞上的分离状况进行比较分析。同时使用 3种细胞共分离到脊灰病毒 (PV)2 0份 ,其中RD细胞分离到 14份 ,L2 0B细胞 16份 ,Hep - 2细胞 8份 ,总分离率为 6 5 %。分离到非脊灰肠道病毒(NPEV) 65株 ,其中RD细胞 3 0株 ,Hep - 2细胞 4 8株 ,分离率为 2 1 1%。结果显示 ,同时使用 3种细胞对提高PV检测灵敏度和NPEV分离率 ,保证脊灰实验室监测质量有重要意义。更多还原

【Abstract】 The L20B cell was formally used in our polio laboratory since July 1998,RD and Hep-2 cells were simultaneously used for acute flaccid paralysis(AFP)cases surveillance 308 samples of AFP cases were inoculated onto 3 types of cells simultaneously The results of virus isolation were compared and analyzed 20 polioviruses in total were isolated,the positive rate was 6 5%,of which 14,16 and 8 were isolated in RD,L20B and Hep-2 cell respectively 65 non-polio enteroviruses(NPEV)were isolated from AFP cases,30 in RD and 48 in Hep-2,the positive rate was 21 1%. Using 3 kinds of cells simutaneously are very necessary for enhancing the sensitivity of AFP virological surveillance,the isolation rate of NPEV,and to ensure the surveillance quality of poliovirus laboratory更多还原