【摘要】 目的　研究新型光敏剂血啉甲醚 (hematoporphyrinmonomethylether,HMME)在肿瘤诊断应用中的光漂白特性。方法　采用组织光学模型对HMME原液在暗室避光条件下进行稀释 ,样品溶液中HMME的浓度分别为 1、2、3、4和 5 μg ml。盛装在计数盘上的样品溶液在Kr+ 激光照射下 ,利用检测装置实时记录反映样品中光敏剂浓度的荧光强度。结果　激发光功率相同时 ,HMME的光漂白时间随着光敏剂浓度的增大而增加。光敏剂浓度相同时 ,其光漂白时间随着激发光功率的增大而减小。无论光敏剂浓度和激发光功率多大 ,光敏剂的光漂白规律都基本一致。实验结果与理论分析相符合。结论　HMME的光漂白特性能满足临床诊断肿瘤的要求 ,是一种性能优良的新型光敏剂更多还原

【Abstract】 Objective To study the photobleaching properties of a new photosensitizer (hematoporphyrin monomethyl ether, HMME) in the diagnosis of tumor. Methods HMME was diluted in optical phantom of tissue up to 1,2,3,4 and 5 μg/ml in darkroom. Solutions of HMME with different concentration were irradiated by Kr + laser in count dishes under different power. Decay of HMME fluorescence intensity in solutions was recorded in maxima at λ=620 nm using the experimental setup fitted with a red sensitive photomultiplier developed by ourselves. Photodynamic and photobleaching properties of HMME in solution were determined from the intensity fluctuations of collected fluorescence. Results The photobleaching time of HMME increased when the concentration of HMME in the solution increased. On the contrary, The photobleaching time of HMME decreased when the power of irradiation laser increased. Regardless of the concentration of HMME and the power of irradiation laser, the photobleaching regularity of HMME is approximately identical. The experimental data show good agreement with the results of theoretical analysis. Conclusions Experimental results show that the photobleaching properties of HMME can satisfy the application for clinic diagnosis of tumors and HMME is also one of the new promising photosensitizer in photodynamic diagnosis.更多还原