【摘要】 目的　分析燃煤型砷中毒患者皮肤癌变过程中增殖细胞核抗原 (PCNA)、B-淋巴细胞瘤 /白血病 - 2抗凋亡蛋白 (BCL- 2 )和突变型 P5 3基因蛋白 (P5 3mt)的表达情况及相关性。方法　免疫组化 ABC法和En Vision TM +System两步法。结果　 PCNA、BCL- 2和 P5 3mt蛋白在砷中毒患者的不同病变皮肤中均有表达 ,且无论是阳性细胞密度还是阳性率均呈现出癌变组 (A组 ) >癌前组 (B组 ) >一般病变组 (C组 )的趋势 ;癌变组 3项指标协同阳性率为 83.33% ,明显高于癌前组的 5 4.5 4%和一般病变组的 2 3.0 8% ;任何两指标间均有高度相关性(P<0 .0 1)。结论　 PCNA、BCL- 2和 P5 3mt的协同高表达是砷性皮肤病变恶性转化的重要标志 ,皮肤角化过度患者有相当比例的表达 ,提示其具有恶变的潜在危险 ;燃煤型砷中毒致皮肤癌变过程中 ,PCNA、BCL - 2和 P5 3mt的异常表达可对砷致皮肤癌的癌前监控和预测提供重要佐证更多还原

【Abstract】 Objective To analyze cooperative expressions of PCNA,BCL 2 and P53 mt proteins in patients’ skin with arseniasis due to coal burning.Methods ABC and EnVision TM +System immuno histochemistry methods were used.Results The expressions of PCNA,BCL 2 and P53 mt proteins were all found in different dermal lesion of arseniasis patients. Moreover, a tendency was presented that both positive cell density and positive percentage were group A>group B>group C. And cooperative positive percentages of the three indexes were 83.33% in group A, which was significantly over group’s B 54.54% and group’s C 23.08%.There was significant correlation between any two indexes(P<0.01).Conclusions In the development of dermal carcinogenesis due to arseniasis from coal burning,the abnormal expressions of PCNA,BCL 2 and P53 mt proteins may supply important evidences in monitor and prevention of dermal cancer from arseniasis.更多还原