【摘要】 目的 :利用双波长分光光度法测定贯叶连翘提取物中金丝桃素的含量。方法 :双波长分光光度法波长组合为λ647nm～λ5 89nm ,甲醇作溶剂空白。结果 :金丝桃素在 1.3 0 μg/ml～ 2 9.75 μg/ml范围内线性好 (r =0 .9998)。平均回收率为 10 2 .3 2 % ,RSD =1.0 8% (n =6)。结论 :结果较为准确、简单易行。更多还原

【Abstract】 Objective: Hypericin content was determined in the extract of hypericum by dual-wavelength spectrophotometry. Methods: The wavelengths were adopeted 589nm～647nm against a methanol blank. Results: The linear correlation was observed within the range of 1.30～29.75μg/ml(r=0.9998). The average recovery of hypericin was 102.32%(n=6), RSD was 1.08%. Conclusion: the result was exact, method was simple and convenient .更多还原