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亲和层析纯化云芝糖肽
Purification of Polysaccharide Peptide of Coriolus Versicolor in Affinity Chromatography
【摘要】 目的 :探讨硼配基亲和层析纯化云芝糖肽的新方法。方法 :采用静态方法研究吸附过程的热力学、动力学、最佳吸附解吸条件 ,依据Chase模型求出最大静态吸附容量qm 和吸附常数Kd。结果 :qm=5 5 .5 7mg/g湿基 ,Kd=5 .312g/L ,动态吸附容量 :43 .1mg多糖 /g湿基 ,10 .3mg蛋白 /g湿基。 结论 :使用亲和层析方法能够初步纯化云芝糖肽
【Abstract】 Objective: To study a new purification method of Polysaccharide Peptide of Coriolus versicolor in affinity chromatography. Methods: Isotherm and kinetics in absorption and the optimal conditions of absorption and elution were studied through static experiments. The static absorption capacity q m and absorption constant K d were calculated according to Chase model. Results: q m =55.57mg/g wet resin, K d =5.312g/L, the dynamic absorption capacity is 43.1mg, polysaccharide/g wet resin and 10.3mg protein/g wet resin.Conclusion: Affinity chromatography can be used to purify PSP preliminarily.
【Key words】 Polysaccharide peptide of Coriolus versicolor; Affinity Chromatography; Separation and purification<;
- 【文献出处】 中成药 ,Chinese Traditional Patent Medicine , 编辑部邮箱 ,2001年01期
- 【分类号】R284
- 【被引频次】9
- 【下载频次】278