【摘要】 目的 :探索用随机扩增多态DNA(RAPD)技术鉴别野山参和栽培人参的可能性。方法 :从经过形态鉴定的野山人参和栽培人参药材中提取DNA模板 ,运用RAPD技术在电泳中产生条带。结果 :用 80个引物 ,其中一个引物产生野山人参可重复的特征条带。结论 :结果证明应用RAPD技术能有效地区别野山人参和栽培人参更多还原

【Abstract】 Objective: To explore the potentiality of random amplified polymorphic DNA (RAPD) technique for the differntiation of wild and cultivated Ginseng.Methods: DNA templated were extracted from wild and cultivated Ginseng of the crude drugs that were identified by morphology, and RAPD technique was applied to produce electrophoresis pattern. Results: Among the 80 primers screened, one was able to generate reproducible band, characteristic RAPD marker band which was unique to each of wild Ginseng samples was identified. Conclusion: The results demonstrated that RAPD marker technique could effectively authenicate wild and cultivated Ginseng samples.更多还原