【摘要】 目的 :观测去势术后大鼠骨组织体视学的改变 ,复制绝经后骨质疏松症的动物模型 ,初步探讨骨质疏松症的发病机制。方法 :将 3 1只 3月龄雌性SD大鼠随机分为卵巢切除术组 (OVX)和假手术组 (sham) ,术后 2 8d和 5 6d分别处死。测量子宫湿重 ,骨矿物密度 (BMD)和骨组织形态计量参数 ,分析骨组织微结构的变化。结果 :OVX组术后 2 8d和 5 6d大鼠子宫湿重和股骨远端 1/ 3处骨密度均显著少于sham组 (P <0 0 5 ) ;OVX组股骨远端和胫骨近端的干骺端骨小梁面积百分率显著少于sham组 (P <0 0 1)。结论 :卵巢切除术后大鼠股骨远端骨矿物密度和骨组织形态计量学参数稳步下降 ,胫骨近端骨矿物密度和骨组织形态计量学参数迅速下降而且不稳定 ;雌激素减少导致的骨质疏松主要发生在长骨的干骺端 ,骨骺受影响较少 ;骨组织形态计量参数中骨小梁面积百分比敏感性和稳定性较高。更多还原

【Abstract】 AIM: To explore the pathophysiological mechanism of postmenopausal osteoporosis, we established an animal model for postmenopausal osteoporosis and investigated the loss of bone mass.METHODS: Thirty-one female SD rats (three months old) were randomly divided into two groups: sixteen ovariectomized (OVX) and fifteen sham-operated group as control. At post-operated day 28, seven rats of either OVX group or sham-operated group were sacrificed. At post-operated day 56, the rest of either were sacrificed. The wet weight of the uteruses were measured and the changes of bone micro-architecture were studied by bone mineral density (BMD) and the data of bone histomorphometry. RESULTS: Compared with the sham group, the mean wet weight and the bone density of proximal tibia 1/3 section of the OVX group was significantly decreased (P<0.05); Data of bone histomorphormetry showed that the trabecular percentage area of metaphysis of both distal femur and proximal tibia was significantly decreased (P<0.01) both at post-operated day 28 and day 56, so were the number of trabecular bone and the thickness of trabecular bone; but no significance was observed in epiphysis.CONCLUSION: BMD and the data of bone histomorphometry at distal femur decreased steadily while those of at proximal tibia did sharply and unsteadily; the early loss of bone mass after ovariectomy was found in metaphysis of long bones.更多还原