【摘要】 目的 :了解透明质酸在大鼠慢性腹膜透析中的作用。方法 :2 0只雄性SD大鼠随机分为 3组。高糖组 (n =8)和透明质酸组 (n =6 )每天分别腹腔内注射 2 5mL的 4 2 5 %葡萄糖腹膜透析液或含 0 0 1%透明质酸 (分子量为 16 0 0kD)的 4 2 5 %葡萄糖腹膜透析液 ,共 7d ;正常对照组 (n =6 ) ,不进行腹腔注射。第 8d进行 4h腹膜功能实验并于不同时点留取血和腹透液样品进行分析。结果 :透明质酸组的腹腔内液容积 (IVP)显著高于高糖组 (P <0 0 1)。高糖组腹腔液体吸收率 (KE)显著高于正常对照组和透明质酸组 ;高糖组和透明质酸组的淋巴液体吸收率(KEB)差异无显著性 ,均显著高于对照组。高糖组的组织间质吸收率 (KET)显著高于对照组和透明质酸组 ,后两者区别不明显。结论 :在大鼠慢性腹膜透析模型中多次重复使用透明质酸 ,可以防止由于长期使用以葡萄糖为渗透压制剂的腹膜透析液所引起的腹腔吸收率增加 ,增加超滤量 ,保护腹膜功能更多还原

【Abstract】 AIM: We have shown that intraperitoneal (IP) addition of hyaluronan could increase peritoneal fluid removal by decreasing peritoneal fluid absorption rate. In this study, we investigated the impact of chronic use of hyaluronan on peritoneal membrane transport characteristics. METHODS: Twenty male SD rats received daily IP injection of 25 mL 4.25% glucose dialysis solution without (HP, n=8) or with 0.025% hyaluronan (HA, n=6) for one week, another six rats did not receive any peritoneal injection. Twenty-four hours after the last injection, a 4h dwell study using 25 mL 4.25% glucose dialysis solution with IP volume marker and frequent dialysate and blood samplings were performed in each rat. RESULTS:IPV was significantly higher in the HA group as compared to the HP group (ANOVA repeated measurement, P<0.01). The peritoneal fluid absorption rate, K E, was significantly increased in the HP group as compared to the other groups. The direct lymphatic fluid absorption rate, K EB , was significantly higher in the two daily infusion groups (HP and HA) as compared to the control group. However, the tissue absorption rate, K ET , was significantly higher in HP group as compared with HA group and control group. CONCLUSION: The present study suggests that repeated intraperitoneal addition of hyaluronan may prevent, at least in part, the membrane damage caused by currently used hypertonic dialysis solution.更多还原