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Canstatin RNA转染对兔血管平滑肌细胞体外增殖的抑制作用
Inhibitory effect of canstatin RNA transfection on the growth of cultured rabbit vascular smooth muscle cells
【摘要】 目的 :探讨canstatin对体外培养的兔血管平滑肌细胞 (VSMC)增殖的影响。方法 :将阳离子脂质体介导的canstatinRNA转染VSMC ,应用细胞计数 ,[3 H]-TdR掺入率测定观察canstatin基因对VSMC增殖的作用。结果 :阳离子脂质体介导的canstatinRNA可有效地转染VSMC并能显著地抑制VSMC的增殖及其DNA的合成率。结论 :Canstatin基因可抑制体外培养的VSMC的增殖
【Abstract】 AIM: To investigate the effect of canstatin on cultured rabbit vascular smooth muscle cells(VSMC). METHODS: By means of cationic liposome mediated method, canstatin RNA was transferred into cultured VSMC. The proliferation quantity of VSMC were determined by the cell counting method and thymidine(-TdR) incorporation. RESULTS: Canstatin RNA could be effectively transferred into cultured primary rabbit aortic smooth muscle cells by the cationic liposome-Dosper and could markedly inhibit VSMC proliferation. CONCLUSION: Transfection of canstatin RNA could inhibit the growth of VSMC in vitro.
- 【文献出处】 中国病理生理杂志 ,Chinese Journal of Pathophysiology , 编辑部邮箱 ,2001年08期
- 【分类号】R363
- 【被引频次】2
- 【下载频次】48