【摘要】 以含绿色荧光蛋白 (GFP)基因的质粒pSK10 0 DS、含切割对虾杆状病毒基因的核酶Rz1的质粒pRGRz1、含核酶Rz2的质粒pRGRz2和转基因空质粒pcDNA3为基础 ,把绿色荧光蛋白GFP基因克隆于pcDNA3的SV40启动了下面 ,由SV40启动子控制 :含四个两种核酸基因的四联体克隆于pcDNA3的多克隆位点区 ,由T7启动子控制 ,构建成含两个Rz1、两个Rz2和GFP基因的转基因质粒pGTR ,以用于转基因抗病毒对虾的研究。更多还原

【Abstract】 The transgenic plasmid of hammerhead ribozymes Rz1 and Rz2 targeting one of the Prawn baculovirus genes has been coustructae.The transgenic plasmid contains double series copies of Rz1 and Rz2 as Rz1-Rz2-Rz1-Rz2.The Green Fluorescent Protein(GFP)gene was selected as the report gene of the transgenic plasmid.The GFP gene was cloned into plasmid pcDNA3 at site 2093bp with Sma I,controlled by SV40 promoter.Ribozyme gene Series was cloned into the MCS of plasmid pcDNA3 with proper restriction endonuclease,controlled by T7 and CMV promoter.The constructed transgenic plasmid was named pGTR and will be used for obtaining transgenic Prawn.更多还原