To study the expression of HCV non structure 5 antigen in vitro , a human HepG2 cell line was incubated with a HCV RNA positive serum. The SABC immunological techniques and gold labeled colloid electron microscopy method were employed to examine for the viral proteins in those cells. The HCV non structure 5 antigen was first detected in the HepG2 cells at 72 hours post incubation. The antigen was continuously observed in the cytoplasm or on the membrane as well on the cell wall of the HepG2 cells even...
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