【摘要】 从建兰花叶病毒 (CyMV)石斛兰分离物中提取病毒RNA ,用反转录———聚合酶链式反应 (RT PCR)方法获得约 5 0 0bp的运动蛋白基因片断 ,插入 pGEM T载体克隆并测序。序列分析表明 ,该基因片断由 474个核苷酸组成 ,和CyMV美国夏威夷分离物、新加坡分离物相应基因核甘酸序列分别具有 97.8%同源性 ;根据核酸序列推导该片断含有 3个部分重叠的开放阅读框架 (ORF) ,分别编码 14kD、12kD和 10kD的多肽更多还原

【Abstract】 The viral RNA was extracted from purified cymbidium mosaic virus (CyMV) isolated from Dendrobium orchid cultivated in Hainan island. The gene of the movement protein (MP) was amplified by means of reverse transcription polymerase chain reaction (RT PCR), and cloned into pGEM T easy vector. Sequence analysis showed that the gene fragment contained 3 open reading frames (ORFs) which may be encoding 14 kD、12 kD and 10 kD peptides. The nucleotide sequence of the cloned gene fragment shared 97.8% homology with the MP genes of CyMV isolated from orchids cultivated in Hawaii and Singapore.更多还原