【摘要】 目的 :　探讨三氧化二镍 ( Ni2 O3)诱导人肺成纤维细胞 ( HLF)恶性转化以及硒和维生素 E的抑制作用。方法 :　在体外用不同浓度的 Ni2 O3处理 HLF细胞 4次 ,利用刀豆凝集素 A( Con A)凝集试验和软琼脂集落形成试验进行转化细胞的恶性鉴定 ;同时添加亚硒酸钠和维生素E,观察其对 Ni2 O3转化作用的影响。结果 :　Ni2 O3可诱导 HLF恶性转化 ,转化细胞生长速度加快 ,排列紊乱 ,失去接触抑制 ,呈交叉重叠生长 ,转化率呈剂量 -反应关系。转化细胞可被低浓度 ConA凝集 ,并在软琼脂内生长。添加硒和维生素 E的处理组与 Ni2 O3组比较 ,转化率显著下降 ( P<0 .0 5 ) ,处理后细胞 Con A凝集反应阴性 ,不能在软琼脂内生长。结论 :　 Ni2 O3有较强的诱导HLF细胞恶性转化的能力 ,具有致癌性。硒和维生素 E对于镍诱导人肺成纤维细胞恶性转化具有抑制作用。更多还原

【Abstract】 Objective: To study the inhibitory effect of selenium and vitamin E on the transformation of human lung fibroblastic cell induced by nickel sesquioxide. Methods: Human lung fibroblastic (HLF) cells were treated four times by Ni 2O 3 of different concentrations in vitro. Meanwhile, Na 2SeO 3 (8 μmol/L) and vitamin E (20 μmol/L) were added into the media containing Ni 2O 3 (6 μmol/L) respectively. The identification of malignancy in transformation of HLF cells was carried out by the tests of Con A and semisolid agar culture. Results: Ni 2O 3 could induce the malignant transformation of HLF cells. The transformed cell proliferated rapidly. The transformed colonies presented an extensively random orientation and the cells were crossed over. The frequency of transformation showed dose response relationship in the experiment. The transformed cells could be agglutinated by low concentration of Con A and grow in semisolid agar. The frequency of transformation of HLF cells exposed to selenium and vitamin E decreased significantly. The cells could not be agglutinated by Con A and could not grow in semisolid agar. Conclusion: Ni 2O 3 can induce strongly the malignant transformation of HLF cells and may be carcinogenic to human beings. The observed inhibitory effect of selenium and vitamin E on the transformation of HLF cells induced by Ni 2O 3 suggests that it is advisable to increase dietary allowance of selenium and vitamin E for people who are exposed occupationally to nickel.更多还原