【摘要】 目的 :　研究不同剂量水平的重组复合纤维 ( AFC)对高脂血症大鼠脂代谢的影响 ,从分子水平探讨 AFC降脂机制 ,寻找安全剂量。方法 :　断乳 SD大鼠 60只 ,雌雄各半 ,经高脂饲料诱导成高脂血症模型后 ,按体重和血浆总胆固醇均衡的原则分为 5组 ,以高脂饲料和正常饲料为对照组 ,其余三组分别饲以不同水平 ( 5%、1 0 %、2 0 % )的重组复合纤维 ,观察重组复合纤维对大鼠血清、肝脏胆固醇及粪胆汁酸代谢的影响 ,应用半定量逆转录聚合酶链反应 ( RT- PCR)观察肝脏Apo- AI基因表达的情况。结果 :　 ( 1 ) 2 0 %的重组复合纤维可显著降低大鼠的体重 ;( 2 )不同剂量水平的重组复合纤维均可显著降低大鼠的血浆总胆固醇 ( TC)和肝脏胆固醇 ( LCH)水平 ,2 0 % AFC的作用最强 ;( 3)大鼠粪胆汁酸的含量与 AFC的剂量水平成反比例 ;( 4 )高剂量水平的重组复合纤维可显著促进肝脏 Apo- AI基因的表达 ,而低剂量水平的重组复合纤维不能刺激其表达。结论 :　各剂量水平的重组复合纤维均具有长期稳定的降脂效果 ,高剂量水平的重组复合纤维降脂作用最强。肝脏载脂蛋白 - AI基因表达增加可能是重组复合纤维降脂机制之一。更多还原

【Abstract】 Objective: To investigate the effect of artificial fiber complexes(AFC)on serum and liver cholesterol levels, and its mechanism in hypercholesterolemic rats.Methods: Male and female weanling SD strain rats 80～100g were divided into five groups, twelve rats per group. As the control groups. group A and B were fed either the normal diet or high cholesterol diet, group C、D、 and E were given one of the diets incorporated with AFC at the levels of 5%、10% or 20% respectively. The experiment lasted three months. By the end of the experiment, the expression of liver Apo AI gene and its regulation by AFC were determined by reverse transcription polymerase chain reaction(RT PCR).Results: (1)20% AFC affected the growth of experimental rats; (2)All levels of AFC significantly lowered the cholesterol levels in plasma and liver, and 20% level was most profound; (3)The amount of fecal bile acid became lower with increasing levels of AFC in diet; (4)The contents of Apo AI mRNA were increased in liver after fed 10%, 20% AFC for three months; in contrast, 5% AFC had no stimulating effect on Apo AI gene expression. Conclusion: All levels of AFC may significantly lower the cholesterol levels of rats, and the effect of high level is more profound. The regulation of Apo AI gene expression in liver may play an important role in the cholesterol lowering effects of AFC.更多还原