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果树组培苗瓶外滤纸桥生根技术研究

Research on Outer Bottle Rooting Technique of Fruit Seedling in Vitro with Filter-paper Bridge

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【作者】 孙仲序刘静王玉军张演义邱治霖

【Author】 Sun Zhongxu 1,Liu jing 2,Wang Yujun 1,Zhang Yanyi 1,and Qiu Zhilin 2 ( 1Department of Horticulture,Shandong Agricultural University, Tai’an 271018; 2Tai’an City Forest Institute, Tai’an 271018)

【机构】 山东农业大学园艺系泰安市林业科研所泰安市林业科研所 泰安271018泰安271000泰安271018泰安271000

【摘要】 对珠美海棠、樱桃colt砧木、弗吉尼亚草莓组培苗进行瓶外滤纸桥生根试验 ,结果表明 :珠美海棠、弗吉尼亚草莓瓶外生根率较瓶内高 2 0 .1%和 4 .1% ;樱桃colt砧木与瓶内生根基本相同 ;瓶外生根时间均缩短至 3~ 10d ;移栽成活率较瓶内分别提高 16 .1%、2 1.4 %和 14 .9% ,达到 93%以上 ;根的活跃面积分别提高 6 .9%、 11.5 4%和 7.5 % ;珠美海棠上表皮、下表皮、栅栏组织厚度及气孔密度分别较瓶内生根苗高 4 5 .8%、 5 0 .3%、 4 7.6 %和 2 .5 % ;该方法可降低成本 75 %以上

【Abstract】 The rooting technique of fruit tissue culture system with filter paper bridge were studied in this experiment.The results indicated that the rooting rates of Zhumei crabapple and strawberry with filter paper bridge were 20% and 4.1% more than those of their controls respectively;the rooting rate of cherry stock was same as that of its control,but the rooting time was decreased to 3-10 days;the transplanting survival rates of these three kinds of fruit tree plantlets all increased up to 93%,the survival rates of Zhumei crabapple,strawberry and cherry stock were increased by 16.1%,21.4% and 14.9% respectively,compared with their controls;the root vigor areas of Zhumei crabapple,Strawberry and cherry stock were increased by 6.9%,11.54% and 7.5%,compared with their controls;the thickness of epicuticle,hypodermis and pajisade tissue and the leaf stoma density of Zhumei crabapple were increased by 45.8%,50.3%,47.6% and 2.5% respectively,compared with its control.This method is simple and convenient.The cost of tissue culture could be cut down 75% than traditional rooting methods.

【基金】 山东省“三零”工程资助项目
  • 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2001年04期
  • 【分类号】S628
  • 【被引频次】45
  • 【下载频次】418
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