【摘要】 根据不同植物LEAFY (LFY)同源基因 3’端序列高度保守性 ,设计合成简并引物 ,采用PCR技术首次从柑桔基因组DNA中分离出一条长 883bp的柑桔LFY同源基因(csLFY)片段。序列分析表明 ,所扩增的 883bpDNA片段中包含一个长 4 76bp的内含子 ,拼接位点与其他植物的LFY同源基因一致。由外显子推导的氨基酸序列与其它植物LFY同源基因的相应区域氨基酸序列同源性为 77%～ 97% ,其中与烟草NFL和矮牵牛ALF的同源性最高 ,达到 97%。RT—PCR研究表明 ,csLFY在柑桔成年结果枝上的花芽和营养芽以及童期幼苗的营养芽中均有表达 ,但童期营养芽中的表达强度明显低于花芽。更多还原

【Abstract】 A pair of degenerate oligonucleotides to highly conserved regions at the 3’terminus of LEAFY(LFY) gene and its homologues,the flower meristem identity genes,is used to amplify a fragment of 883bp by PCR from the citrus genomic DNA.Sequencing indicated that there is an intron of 473bp in the fragment.The splice donor site and the splice acceptor site are in identical position to the LFY gene and its homologues.The amino acid sequence deduced from the exons is 77%-97% homologous to that of the corresponding regions of LFY and its orthologues.The expression patterns of the citrus LFY homolog( csLFY )were analyzed by RT PCR.The result indicates that csLFY expression was detected in vegetative and flower buds from the adult tree as well as vegetative buds from the several month old seedlings.However,the level of the csLFY transcripts in the juvenile buds is lower than in the flower buds.更多还原