【摘要】 以大白菜自交系AB 81的子叶和真叶切块为外植体 ,建立了高频不定芽离体再生系统。在MS附加TDZ 0 .2mg/L ,NAA 2 .0mg/L ,AgNO310mg/L和ABA 0 .2 5mg/L的再生培养基上 ,子叶和真叶的不定芽再生频率分别达到 93.3%和 90 .0 % ,每块外植体的不定芽数达 3～ 6个 ,最多达 2 1个。以EHA10 5/pMOG4 10为载体转化侵染大白菜AB 81的子叶 ,获得较高gusA基因瞬时表达频率的条件为 :子叶预培养 2～ 3d ;侵染的工程菌液的浓度OD 0 .3～ 0 .5;侵染时间 2～ 10min ;共培养时间 2～ 3d更多还原

【Abstract】 An efficient shoot regeneration system was established using cotyledon and leave cuts of the inbred line‘AB 81’as explants.On the MS medium supplemented with 10mg/L AgNO 3,0.2mg/L TDZ,2mg/L NAA and 0.25 mg/L ABA,the regeneration frequency of the cotyledon and leave explants was up to 93.3% and 90% respectively.The mean number and the most number of shoots per explant was 3-6 and 21 respectively.Cotyledons of AB 81 were inoculated with Agrobacterium tumefaciens strain EHA105 containing pMOG410 carrying gus A intron gene.The optimum conditions for the transient expression of gus A gene were as follows:preculture time of 2-3 days,bacterium density of OD0.3-0.5,infection time of 2-10min,and coculture time of 2-3 days.更多还原