【摘要】 以 pBS+为克隆载体 ,构建圈养大熊猫基因组DNA的小插入文库 .分别用生物素标记的 (CAC) 5和γ - 32 P -dATP标记的 (CA) 15寡核苷酸为探针 ,对重组质粒进行斑点杂交 ,获得 15个阳性克隆 ,并测定了插入片段的DNA序列 .其中有一部分序列已被分析 ,本文对剩余的 8个DNA序列进行分析 ,发现它们大小不一 ,从 2 5 9bp～ 881bp ,有 6个序列含有不同重复单位的一、二、四核苷酸的完整串联重复 .斑点杂交和Southern杂交证明 ,这些序列均来自大熊猫基因组 .上述结果为利用PCR技术研究大熊猫遗传多样性和了解大熊猫基因组结构积累资料 .表 2参 15更多还原

【Abstract】 The small- insert genomic DNA library was constructed from giant panda genome. Cloning vector pBS + and 205 recombinant plasmids selected were hybridized to dot blotting with probes, γ- 32 P-dATP-labeled (CAC) 5 and biotinylated (CA) 15 , respectively. As a result, 15 positive clones with repeat insert were screened and sequenced. After analysis of the sequences of 8 microsatellites, it was found that they were different in length ranging from 259 bp to 881 bp, and 6 of them possessed single, di-, tetra-nucleotide repeat. Dot and Southern hybridizations showed that these DNA sequences come from giant panda genome.It provides the basis for studying heriditary polymorphism and genomic structure of giant panda with PCR technique. Tab 2, Ref 15更多还原