【摘要】 目的　观察红花黄酮成分杨梅素 (Myr)和山奈酚 (Kae)对血小板激活因子 (PAF)诱导的兔洗涤血小板聚集、5 HT释放及血小板内游离钙离子浓度升高的影响。方法　以比浊法测定家兔洗涤血小板 (WRP)聚集 ,邻苯二甲醛 (OPT)荧光法测定 5 HT浓度 ,Fura 2荧光探针测定血小板内游离钙离子浓度。结果　Myr和Kae体外呈浓度依赖性地抑制PAF诱发的WRP聚集及 5 HT释放。Myr抑制WRP聚集的IC50 为 17 5 μmol·L- 1 ;抑制 5 HT释放的IC50 为6 4 1μmol·L- 1 。Kae抑制聚集、释放作用的IC50 分别为 73 7μmol·L- 1 和 12 8μmol·L- 1 ;同时Myr和Kae均能明显抑制PAF引起的血小板内游离钙增高。结论　Myr和Kae可抑制PAF诱导的血小板活化作用更多还原

【Abstract】 AIM To observe the inhibitory effect of myricetin (Myr) and kaempferol (Kae) (components of Carthamus tinctorius L.) against washed rabbit platelet (WRP) aggregation, 5 HT release and intercellular free calcium concentration elevation induced by platelet activating factor (PAF). METHODS The platelet aggregation induced by PAF was determined with turbidity assay. The platelet 5 HT release and platelet inner free calcium concentration elevation induced by PAF were measured by OPT fluorescence assay and Fura 2/AM probe fluorescent technique respectively. RESULTS The WRP aggregation and 5 HT release induced by PAF (9 55×10 -9 mol·L -1 ) were both inhibited by Myr and Kae in a concentration dependent manner. WRP aggregation rate can be significantly decreased by Myr and Kae. Low concentration Kae (21 8 μmol·L -1 ) showed no inhibition effect against platelet aggregation. The IC 50 of Myr and Kae against WRP aggregation were 17 5 μmol·L -1 and 73 7 μmol·L -1 respectively. It was shown by OPT fluorescence assay that Myr (7 86 μmol·L -1 ) and Kae (21 8 μmol·L -1 ) were not effective against platelet 5 HT release ( P >0 05). The IC 50 of Myr was 64 1 μmol·L -1 and that of Kae was 128 μmol·L -1 . It was also found that the platelet inner free calcium concentration elevation evoked by PAF (4 78×10 -10 mol·L -1 ) was inhibited by Myr and Kae. CONCLUSION The rabbit platelet activation induced by platelet activating factor can be inhibited by Myr and Kae.更多还原