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脉冲式破伤风类毒素聚乳酸微球动物免疫效果研究

LONG TERM IMMUNOGENICITY AND IMMUNOLOGIC PRIMING OF ANIMALS TO PULSED CONTROLLED-RELEASE SYSTEM FOR TETANUS TOXOID VACCINE DELIVERY

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【作者】 何应陈志华魏树礼

【Author】 HE Ying 1, CHEN Zhi-hua 2, WEI Shu-li 1 (1. School of Pharmaceutical Sciences, Peking University, Beijing 100083, China; 2. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100850, China)

【机构】 北京大学药学院!北京100083军事医学科学院微生物流行病研究所!北京100850

【摘要】 目的 研究破伤风类毒素聚乳酸微球在动物体内的免疫效果及影响因素。方法 观察破伤风类毒素聚乳酸微球免疫动物后 1年中 ,动物血清抗体反应及免疫记忆反应的规律。结果 微球中抗原释放为脉冲式 ,释放时间与聚乳酸分子量、微球载药量及粒径有关 ,微球引起的抗体反应显著高于破伤风类毒素溶液一次免疫 ,与溶液 3次免疫效果相似。结论 创制了一种新型脉冲式释药系统 ,一次注射完成全程免疫 ,有巨大的应用价值

【Abstract】 AIM To evaluate the immunogenicity of tetanus toxoid (TT) encapsulated in biodegradable polymer microspheres composed of polylactide (PLA). METHODS The antibody levels elicited by microsphere formulations in mice for 1 year were examined, the anamnestic responses to a low dose booster 1 year after priming were also examined. RESULTS Microsphere formulations made from PLA were characterized by pulse controlled-release models, differing in polymer molecular weight, protein loading and particle size of the microspheres. Microsphere formulations elicited significantly higher IgG antibody levels than a single injection of soluble TT. The antibody levels elicited by microsphere formulations were similar to those elicited by three doses of soluble TT. CONCLUSION A single-dose tetanus toxoid based on pulsed release from biodegradable and biocompatible polymer microspheres has been developed. The formulations showed great benefits and pharmaceutical application.

【关键词】 脉冲破伤风类毒素微球动物免疫
【Key words】 pulsetetanus toxoidmicrospheresanimals
【基金】 国家自然科学基金资助项目 ( 39770 878)
  • 【文献出处】 药学学报 ,Acta Pharmaceutica Sinica , 编辑部邮箱 ,2001年09期
  • 【分类号】R186
  • 【被引频次】18
  • 【下载频次】132
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