【摘要】 目的　研究中药灯盏花 [Erigeronbreviscapus (Vant.)Hand Mazz]的心血管活性成分。 方法　用各种色谱技术进行分离 ,用IR ,UV ,MS ,1HNMR ,13CNMR ,2DNMR光谱鉴定他们的结构。以乳酸脱氢酶 (LDH)释放量为指标测定BCMEC(bovinecerebralmicrovascularendothelialcell)损伤 ,比色法测定药物体外抗氧化和抗活性氧能力。结果　从灯盏花中分离得到 2个化合物 ,分别鉴定化合物的结构为 :1 O 甲基 3 ,5 O 双咖啡酰基奎宁酸甲酯 (III)和 5 O 咖啡酰基奎宁酸丁酯 (IV)。结论　化合物III,IV为新的酚酸类化合物 ,化合物III对LPC引起的BCMEC损伤有明显的保护作用更多还原

【Abstract】 AIM To isolate and identify the active constituents of Erigeron brevisapus (Vant.) Hand. Mazz. METHODS The chemical constituents were isolated by silica gel column chromatography and two new compounds were obtained. Their structures were elucidated by IR, MS, 1HNMR , 13 CNMR , DEPT and 2DNMR. The injury of BCMEC (bovine cerebral microvascular endothelial cell) was determined by lactic dehydrogenase (LDH), the ability of the drugs anti oxidation and scavenging oxidation of free radical was measured by colorimetric method. RESULTS Two new compounds have been identified as 1 O methyl 3, 5 O dicaffeoyl quinic acid methyl ester (III) and 5 O caffeoyl quinic acid butyl ester (IV). CONCLUSION Compounds III and IV are new compounds. Compound III can protect BCMEC injury by LPC.更多还原