【摘要】 目的:研究中性粒细胞在外源病原微生物金黄色葡萄球菌及其分泌物刺激而免疫激活状态下的粘附特性,考察不同的细菌浓度对中性粒细胞粘附特性的影响。材料与方法:采用微管吸吮技术测量大鼠中性粒细胞经不同浓度金黄色葡萄球菌及其分泌物刺激后与内皮细胞的粘附特性。结果:中性粒细胞经金黄色葡萄球菌免疫激活后与内皮细胞的粘附力和对照组比较均显著增高,但随着细菌浓度的提高而表现出饱和效应;用金黄色葡萄球菌分泌物(培养液)处理中性粒细胞后,其与内皮细胞的粘附力和对照组比较却有所降低。将经细菌处理后的中性粒细胞分离出再与内皮细胞黏附,黏附力增加幅度更大。结论:中性粒细胞经金黄色葡萄球菌免疫激活后粘附分子表达增加,但金黄色葡萄球菌分泌物可能对中性粒细胞粘附分子表达起抑制作用。在此过程中,内皮细胞可能受到金黄色葡萄球菌及其分泌物的损伤。更多还原

【Abstract】 purpose:Study the adhesive properties of rat polymorphonuclear leukocyte(PMN)after stimulated with different concentration staphylococcus aureus and its secretions on endothelial cells.Material and methods:A micropipette aspiration technique was adopted to investigate the adhesive force of rat PMN on human umbilical vein endothelial cells(HUVECs).Results:it showed that the adhesive force of PMNs on HUVECs are higher than that of control after stimulated by staphylococcus aureus.Further,they showed a staturated phenomenon after the concentration of staphylococcus aureus increased.But the adhesive force of PMNs on HUVECs are lower compared with control after treated with secretions solution of staphylococcus arueus.the adhesive force increased more while there were no staphylococcus arueus.Conclusion:when PMN were stimulated by staphylococcus aureus,the expression of its adhesive molecules increased;but the secretions of staphylococcus aureus maybe inhibit the expression of its adhesive molecules.Perhaps HUVECs were damnified by staphylococcus aureus and its secretions solution in the course of this adhesion.更多还原