【摘要】 [目的 ]了解山西省特殊人群中丙型肝炎病毒 (HCV )的流行现状和基因分布规律。 [方法 ] 1997～ 1999年采集山西省妇女教养所劳教人员、性病门诊病人和饮食服务行业从业人员血清 14 87份 ,用ELISA法检测抗 -HCV ,抗体阳性的 42份标本进一步用型特异性引物RT -PCR进行基因分型 ,并对 3份标本进行了PCR产物的直接序列测定。[结果 ] 14 87份标本中 ,妇女教养所劳教人员、性病门诊病人和从业人员的抗 -HCV检出率分别为 4 49%、2 17%、0 95 % ,三者间的差异有统计学意义。但妇教所劳教人员中有性伴侣和无性伴侣者间 ,性病门诊的性病患者和非性病患者间的检出率差异无统计学意义。选择 42份抗 -HCV阳性标本进行HCV基因分型 ,仅检出了Ⅱ / 1b型、Ⅲ / 2a型和Ⅱ /Ⅲ混合型。 3份标本HCV核心区部分核苷酸序列与日本株相应区段的差异均为 6 % ;1份Ⅱ / 1b型与河北株的差异大于与日本、上海和湖南株的差异。 [结论 ]HCV感染与 1年内有 1个以上性伴侣或患性病的关系不密切 ;性乱者与性病患者的HCV基因序列与其他地区的差别不大更多还原

【Abstract】 Objective]To investigate the HCV prevalence and genotypes in special population in Shanxi Province from 1997-1999 [Methods]HCV antibody was detected in 1 487 sera collected from woman penitentiary of Shanxi, STD clinical patients and workers in public place by ELISA 42 HCV antibody positive sera were further genotyped by type-specific PCR, and direct sequencing of PCR products were conducted on three genotyping positive samples [Results]The positive rate of HCV antibodies for woman of penitentiary, patients of STD clinic and workers in public place are 4 49%, 2 17%, 0 95% respectively, which have significant difference There was no significant difference of HCV antibodies between penitentiary women who had more than 1 partner during 1 year and who had no partner The same result was also found in STD clinic between STD patient and non-STD patient Only genotype Ⅱ/1b,Ⅲ/2a and Ⅱ/ Ⅲ mix infection were detected Part of nucleotide sequence in HCV core region of tree samples had 94% homogenous with Japan strains of Ⅱ/1b and Ⅲ/2a The nucleotide difference inⅡ/1b sample from Hebei is more than Japan, Shanghi and Hunan [Conclusions]The infection of HCV may be not related to fact or of more than 1 partner in 1 year or STD The nucleotide sequence for HCV in multisexual individuals or STD patients has no significant difference from that of other regions更多还原