【摘要】 目的··:建立生物检材中吗啡的薄层色谱扫描 (TLCS)分析方法 ,研究急性染毒家兔体内吗啡的分布情况。方法··:组织检材和尿液经碱性高压水解后用氯仿/异丙醇 (9∶1)提取 ,TLCS法定性定量分析。结果··:碱性高压水解尿液和肝脏中吗啡的提取回收率大于80 % ,精密度小于9.3 %。急性染毒家兔尿液和胆汁中吗啡含量最高,肝、肾中次之,脑和血清中最低。结论··:肾、胆是吗啡的主要排泄途径 ,对疑为海洛因、吗啡中毒延缓死亡的案件 ,尿液、胆汁、肝和肾是毒物分析的最佳检材,建立的TLCS法可用于体内吗啡的检测更多还原

【Abstract】 Objective: To establish a method of thin layer chromatography scan(TLCS)and study the distribution of morphine in the body of acute poisoning rabbits. Method: Samples were alkali hydrolyzed by high pressure and extracted with chloroform/isopropanol (9:1). Morphine concentration was analyzed by TLCS. Result: The recovery rate of morphine was over 80% and the related standard deviation was less than 9.3%. The concentration of morphine in urine and bile was the highest, while the concentration in brain and serum was lower than that in other tissues .Conclusion: Kidney and gallbladder are major ways of morphine excretion. Urine or bile samples can be used for toxicological analy_ sis in case of suspecting heroin or morphine poisoning. TLCS is suitable for the determination of morphine in body.更多还原