【摘要】 通过对不同培养基及不同C源、N源、接种量等影响因素的单因素多水平实验 ,确定了最佳发酵工艺 :以5 %麸皮水 ,1%玉米浆为培养基 ,初始pH 4 0 ,种龄为 10h ,接种量 5 % ,装液量 10 0mL/ 5 0 0mL三角瓶 ,16 0r/min ,30℃ ,发酵 48h。在优化后的条件下 ,培养基的成本及制作时间均降低 ,而且总活力提高为 140U/mL。更多还原

【Abstract】 This paper mainly dealed with the fermentation condition of antibiotic substance from Rhizopus Chinese No12 By single factor experiments,the optimum fermentation condition had been determined,which were the optimum medium(5%bran +1% corn steep liquid),the initial pH value of fermentation medium(pH4 0)and the seed age(10h),volume of inoculation(5%),the volume of medium in 500ml flask(100ml),shaking speed(160r/min),culture temperature(30℃)and culture time(48h) Under the optimum condition,the price and preparing time for the medium were decreased,and the total active units were increased to 140U/ml更多还原