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传染性法氏囊病病毒结构蛋白VP2比较及高表达毒株的分离
Comparison and Isolation of Infectious Bursal Disease Viruses(IBDV) Highly Expressing Viral Structural Protein VP2
【摘要】 对 2 9个传染性法氏囊病病毒 (IBDV)的病理组织和毒株分别增殖后 ,采用不连续蔗糖密度梯度离心的方法纯化病毒 ,经SDS PAGE分析 ,病毒结构蛋白VP2在不同毒株表达量有较大差异。对其中 0 0ZB和99TA32个VP2相对高表达的IBD病理组织样品进行病毒分离 ,获得了 2个IBDVVP2相对高表达毒株
【Abstract】 29of pathological tissues and isolates of infectious bursal disease virus (IBDV) were propagated in SPF chicken, embryo and embryo fibroblast(CEF) respectively. The viruses were purified by sucrose discontinuous gradient ultra centrifugation and analyzed with SDS PAGE .The result showed that the expression of viral structural protein VP2 were appreciable diversity among the IBDV. Two isolates of IBDV were separated from pathological tissue samples of 00ZB and 99TA3.
【关键词】 传染性法氏囊病病毒;
结构蛋白VP2;
分离;
【Key words】 Infectious bursal disease virus; Viral structural protein VP2; Isolation;
【Key words】 Infectious bursal disease virus; Viral structural protein VP2; Isolation;
【基金】 江苏省应用基础研究项目资助 (BJ990 49)
- 【文献出处】 药物生物技术 ,Pharmaceutical Biotechnology , 编辑部邮箱 ,2001年04期
- 【分类号】R373
- 【被引频次】1
- 【下载频次】32