【摘要】 目的 :建立一种用高效液相色谱法测定呱仑酸钠的含量及有关物质的方法。方法 :在InertsilCN - 3柱上 ,以磷酸盐缓冲液 (pH =7) -乙腈 (5 0∶5 0 )为流动相 ,于 2 90nm波长处检测 ,流速为 1 0mL·min-1。结果 :呱仑酸钠的浓度在1 0～ 6 0 μg·mL-1范围内线性关系良好 ,回归方程 :A =6 2 0 0× 10 5C +6 374× 10 4 ,r =0 9996。精密度试验RSD =0 2 9% (n =6 ) ,最低检出限为 0 2 4ng。有关物质可检出 2个杂质。结论 :本方法简单、快速、结果准确更多还原

【Abstract】 Objective:A HPLC method was established for the determination of sodium gualenate and its related substances.Method:The chromatographic condition were:Inertsil CN-3 5 μm (4 6 mm×250 mm);phosphate buffer (pH 7 0)-acetonitrile (50∶50) as mobile phase;flow rate was 1 0 mL·min -1 ,wavelength of detector was 290 nm.Results:The standard curve was linear in the range of 1 0～6 0 μg·mL -1 and the correlation coefficient was 0 999 6.The precision of sodium gualenate was good (RSD=0 29%, n= 6).Conclusion:The proposed method is simple,rapid,accurate and reliable.更多还原